THE ACTION OF COLEUS AROMATICUS AS A POTENTIAL WOUND HEALING AGENT IN EXPERIMENTALLY INDUCED DIABETIC MICE

Abstract

The ethanolic and aqueous extract of Coleus aromaticus leaves and roots were evaluated for its
wound healing activity in diabetic mice induced experimentally with Monosodium Glutamate
(MSG) via excision and dead space wound models with the application of 100 mg kg-1 of body
weight of extract. Excision wound was created on five different groups of mice and the wound
contraction was measured for ten uninterrupted days. Dead space wound model was performed
on four different groups of mice for the estimation of the weight of both wet and dry
granulation tissue followed by the histological investigation of the wet granulation tissue after
ten days. Ethanolic extract treated wounds showed 76.6% of wound area reduction compared to
the controls that only showed 55.9 % of wound area reduction. The rate of epithelialization in
ethanolic extract treated mice was interestingly higher compared to the controls. Histological
investigation shows an increase in granulation tissue, good proliferation of collagen tissue and
satisfactory angiogenesis in the leaves and root extract treated mice compared to the controls.
Coleus aromaticus stimulates significant wound healing in experimentally induced diabetic
mice and evaluation of the effectiveness of Coleus aromaticus as wound healing agent on
human organism is highly suggested.